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Hydroxysterols such as 22R-OH-cholesterol bypasses the action of StAR, providing an index of the maximal possible level of steroidogenesis that these cells can generate. An increase in the expression of the marker of steroidogenesis, 23β-HSD-CoA (23β-HSD-CC), is associated with activation of the transcription factor ERK1/2, and this enzyme is also found in the hypothalamus [27]. Thus, the increased expression of ERK1/2 after steroid application increases the synthesis of the gonadotrogen DHT and th